crispr

Number of posts: 13

Edit single bases with Benchling!

Currently, scientists can edit genes, with CRISPR, but how well can this method edit a specific base? Not very well — only 0.1% to 5%, and at best, 20% [1-3]. That’s all about to change! Alexis Komor and others from David Liu’s lab at Harvard recently devised a CRISPR mechanism, called ba…

crispr, how-to guide

Introducing an optimized score for sgRNA design

When designing sgRNAs, selecting an sgRNA that can cleave your targeted genomic region effectively (i.e. high on-target activity) is very important. How can you predict which sgRNAs are most likely to work well for your experiments? Introduction Benchling just released an optimized…

crispr, Product

Rule Set 2: the Science Behind the Metric

In 2014, Doench et al. developed the “on-target score” for measuring how efficient a given sgRNA is at guiding Cas9 to the correct spot for cleaving. The score has helped scientists select better sgRNAs for CRISPR experiments. In February 2016, Doench, Fusi et al. published an improved algori…

crispr

How to express CRISPR in your target cells

This tutorial is part 4 of 6 in the series “How to Create Knockouts Using CRISPR.” In the previous post, we talked about how to synthesize gRNA and Cas9 based on what cell line you will use. In part 4 of this tutorial, we discuss different delivery methods for your gRNA and Cas9. Intro…

how-to guide, crispr

Design HR templates for CRISPR quickly and accurately

Thousands of scientists use Benchling's CRISPR tools to design and score guides. Today, we're excited to announce an addition to our CRISPR tools: design of HR templates for knock-in experiments. We also now support 50+ genomes and are actively adding support for more genomes as scientists request t…

Product, crispr

How to Synthesize your gRNAs for CRISPR

This tutorial is part 3 of 6 in the series “How to Create Knockouts Using CRISPR.” To edit a cell’s genome, you need to co-express both guide RNAs (gRNA) and the RNA-directed DNA cleaving enzyme Cas9 into the cell. In previous posts, we discussed how to select the best Cas9 for your applic…

how-to guide, crispr

How to Design gRNAs to Target Your Favorite Gene

This tutorial is part 2 of 6 in the series “How to Create Knockouts Using CRISPR.” OK. Now we have decided the cell line and Cas9 for our experiments. Time to start designing gRNAs! To eliminate the function of your favorite gene, you want to target a consecutively-expressed exon n…

how-to guide, crispr